β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation

Figure 9 Protein expression of cells exposed to IPA-3 and IPA-3 plus IR examined by western blotting. A: Validation of phospho-Pak1 downregulation in response to IPA-3 treatment, using GAPDH as a loading control. IPA-3 downregulated phospho-Pak1 (T423) and its downstream p-ERK1/2; B: IPA-3 pretreatment decreased IR-induced upregulation of phospho-Pak1 (T423) and phospho-ERK1/2. The level of cleaved caspase-3 was consistent with the level of apoptosis at the cytological level (Figure 8B).