Mechanisms of regulation of PFKFB expression in pancreatic and gastric cancer cells

Figure 12 Representative polyacrylamide gel autoradiograph employed in a typical ribonuclease protection assay of endogenous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 mRNA in pancreatic carcinoma cell line Panc1, stable transfected by pcDNA3. 1(+) vector (Panc1 cells) or by dominant/negative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (Panc1 + dnPFKFB-3) in normoxic (N) condition and after treatment of Panc1 cells with dimethyloxalylglycine, inhibitor of prolyl hydroxylase (I; 1 mmol/L for 6 h). The 18S rRNA antisense probe was used as control of analyzed RNA quantity[89]. PFKFB: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.