CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation

Figure 2 Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10^-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10^-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.