Understanding the interaction of hepatitis C virus with host DEAD-box RNA helicases

Figure 2 Analysis of protein expression following knockdown of endogenous DDX5, DDX3 and DDX6 in Huh7. 5 cells. A: Cells transfected with 40 nmol/L of DDX5 short interfering RNA (siRNA) or a control siRNA; B: Cells transfected with 40 nmol/L of DDX3 siRNA or control siRNA; C: Cells transfected with 40 nmol/L of DDX6 siRNA or control siRNA. At 48 h, cells were infected with J6/JFH-1 (p47) hepatitis C virus (HCV) at multiplicity of infection of 2 as previously described[72]. Cell lysates were prepared 0, 2, 4 d post-infection (d.p.i.) and subjected to immunoblotting with the respective primary antibodies. Expression of NS5B was used to confirm HCV infection and β-actin served as a loading control. Three independent experiments were performed and one representative set of data is shown.