Nrf2 and Snail-1 in the prevention of experimental liver fibrosis by caffeine

Figure 3 Expression of inflammatory genes in liver. A: Immunohistochemistry for CD11b, sections 4 μm thick, stained with Masson’s trichrome, × 40. Results for CD11b positive area are shown as percentage; B: Tumor necrosis factor alpha (TNF-α) liver levels in different groups of treatment, performed by enzyme-linked immunosorbant assay; C-E: Reverse transcription-polymerase chain reaction were performed for TNF-α, interleukin-1β (IL-1β), IL-6. Gene amplification was normalized against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. TAA: Thioacetamide; BDL: Bile duct ligation. CFA: Caffeine.