Nrf2 and Snail-1 in the prevention of experimental liver fibrosis by caffeine

Figure 1 Macroscopic and histological differences and fibrosis index with caffeine treatment. A: Liver fibrosis percentage of healthy rats, caffeine (CFA)-treated, thioacetamide (TAA)-intoxicated, TAA-intoxicated treated with CFA, bile duct ligation (BDL) rats, BDL rats treated with CFA. Sections, 4 μm thick, stained with Masson’s trichrome, × 10. White arrows show the extracellular matrix (ECM) (fibrosis); B: Fibrosis quantification. Fibrosis percentages are shown, they were obtained by computer-assisted morphometric analysis (Software Image pro plus 6.3); C: Knodell Index for fibrosis, sections 4 μm thick, stained with Masson’s trichrome, × 10; D: Inflammatory infiltrate amount. Sections 4 μm thick, stained with hematoxylin and eosin, × 40. Black arrows show inflammatory cells; E: Knodell Index for fibrosis, sections 4 μm thick, stained with hematoxylin and eosin, × 40.