Synergistic effect of interleukin-10-receptor variants in a case of early-onset ulcerative colitis

Figure 2 Real time polymerase chain reaction analysis of interleukin-10 receptors and COX2 performed on peripheral blood cells. A: Copy number quantification of interleukin-10 (IL10) gene. Real time polymerase chain reaction (PCR) quantification analysis was performed for IL10RA. IL10RA-exon4: Amplified fragment at the boundaries of exon 4 and IVS4 of the gene; IL10RA-exon7: Amplified fragment at the boundaries of exon 7 and IVS7 of the gene; Patient numbering corresponds to that adopted in the pedigree shown in Figure 1A. B: Real time PCR quantification analysis of IL10RA and IL10RB mRNA. Real time RT-PCR quantification analysis was performed for IL10RA and IL10RB mRNA. C1-8: Mean value between all healthy samples used as calibrator to measure the relative expression; C1 to C8: Healthy subjects. Patient numbering corresponds to that adopted in the pedigree shown in Figure 1A. C: Real Time PCR quantification analysis of COX2 messenger. H1-5, H6-10, H11-15: Mixes of healthy subjects; Hm: Mean value between all healthy samples used as calibrator to measure the relative expression; Patient numbering corresponds to that adopted in the pedigree shown in Figure 1A.