Basic transcription factor 3 is involved in gastric cancer development and progression

Figure 2 Silencing of basic transcription factor 3 using small interfering RNA. A: Transfection rates of small interfering RNA (siRNA)-normal control (NC)/siRNA-basic transcription factor 3 (BTF3)-green fluorescent protein (GFP) vectors in human SGC7901 cells, estimated by bright field and fluorescence microscope; B: Bar charts represent quantitative real-time polymerase chain reaction data of BTF3 mRNA levels in control and BTF3-siRNA transfected SGC7901 cells (^bP < 0.01 between groups, unpaired t test); C: Immuno-blotting analysis using BTF3-specific antibodies to detect BTF3 protein expressions in control and BTF3-siRNA transfected SGC7901 cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as internal control.