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World J Gastroenterol. Jul 28, 2013; 19(28): 4495-4503
Published online Jul 28, 2013. doi: 10.3748/wjg.v19.i28.4495
Published online Jul 28, 2013. doi: 10.3748/wjg.v19.i28.4495
Basic transcription factor 3 is involved in gastric cancer development and progression
Qi Liu, Zhong-Cheng Huang, Hong-Yu Dong, Guang-Yi Li, Ke Zhou, Department of Anorectal Surgery, People’s Hospital of Hunan Province, the First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan Province, China
Jian-Ping Zhou, Department of Elderly Surgery, the Second Xiangya Hospital of Central South University, Changsha 410077, Hunan Province, China
Bin Li, Department of Tumor Chemotherapy, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
Shao-Lin Nie, Department of Gastrointestinal Surgery, Hunan Provincial Tumor Hospital, the Affiliated Tumor Hospital of Central South University, Changsha 410013, Hunan Province, China
Author contributions: Liu Q and Nie SL conceived and designed the study; Liu Q, Zhou JP, Li B, Huang ZC, Dong HY and Nie SL acquired data; Zhou JP, Li GY and Zhou K performed the data analysis; all authors have read and approved the final version of the manuscript.
Supported by Science and Technology Project of Hunan Province, China, No. 2013FJ3151
Correspondence to: Shao-Lin Nie, MD, Professor of Medicine, Deputy Chief, Department of Gastrointestinal Surgery, Hunan Provincial Tumor Hospital, the Affiliated Tumor Hospital of Central South University, No. 582 Xianjia Lake Road, Changsha 410013, Hunan Province, China. nieslbm@163.com
Telephone: +86-731-89762121 Fax: +86-731-88651999
Received: January 21, 2013
Revised: April 25, 2013
Accepted: June 1, 2013
Published online: July 28, 2013
Revised: April 25, 2013
Accepted: June 1, 2013
Published online: July 28, 2013
Core Tip
Core tip: After we found that basic transcription factor 3 (BTF3) transcription rates in human gastric tumor tissue samples were significantly higher than in adjacent normal tissues, we extended our study on gastric cancer cell lines. We silenced BTF3 in SGC7901 cells, which led to 82%, 70%, 57%, 49% and 44% proliferation rates of the control within the first 5 d after infection with a small interfering RNA-BTF3 containing lentivirus. After BTF3 silencing, the percentage the G1 phase arrested SGC7901 cells was decreased (P = 0.000), the percentages of cells in the S (P = 0.031) and G2/M (P = 0.027) phases were increased and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014).