Intestinal injury can be reduced by intra-arterial postischemic perfusion with hypertonic saline

Figure 1 The sequential steps of the intestinal perfusion in vivo. A: The abdominal aorta (aa) is occluded by a clip, followed by cannulation of the vessel; the superior mesenteric artery (SMA) is clipped; B: The aortic clip is positioned above the SMA; the cannula is positioned at the level of SMA to allow its perfusion with hypertonic saline; the perfusate outflow is secured through the opened ileocecal branch (ib) of the superior mesenteric vein (SMV); the main trunk of SMV is clipped; C: The cannula is removed to allow blood flow through the SMA to resume simultaneously with the following procedures: the aortic clip is positioned as in Figure 1A; the clip occluding the SMV is removed, and bleeding from the ileocecal branch of the SMV is prevented by ligation.