8-Hydroxydeoxyguanosine: Not mere biomarker for oxidative stress, but remedy for oxidative stress-implicated gastrointestinal diseases

Figure 3 Ameliorating effect of exogenous 8-hydroxydeoxyguanosine against dextran sodium sulfate-induced colitis. Dextran sodium sulfate (DSS) administration resulted in moderate to severe colitis manifested as significantly shortened colon length. However, colon length was significantly preserved by exogenous administration of 8-hydroxydeoxyguanosine (8-OHdG) (see arrows to compare the colon length), as shown by the pathological observation that the degree of DSS-induced colitis was apparently improved, suggesting that exogenous 8-OHdG had a significant preventive effect against DSS-induced colitis[47]. Eighteen mice were divided into three groups of six: a non-treated control group (Normal group); 5% DSS in tap water ingestion for 1 wk (DSS group); and DSS with daily injection of 8-OHdG (DSS + 8-OHdG group). 8-OHdG powder was dissolved in PBS and the Normal and DSS groups were treated as a negative control. Clinical phenotypes such as hematochezia and rectal prolapse were investigated and charted daily. There was no mortality observed in any of the groups. After 7 d DSS ingestion, all mice were killed and colons were removed, opened longitudinally, and rinsed with phosphate buffer solution. The lengths of colon were measured, and isolated tissues were subjected to histological examination.