Mutual regulation between microRNA-373 and methyl-CpG-binding domain protein 2 in hilar cholangiocarcinoma

Figure 6 miR-373 negatively regulates methyl-CpG-binding domain protein expression through binding to three prime untranslated region. A: miRNA target prediction screened one computative miR-373 binding site at methyl-CpG-binding domain protein (MBD)2-three prime untranslated region (3’UTR); B: 3’UTR luciferase reporter assay showed a reduction of relative luciferase activity of wild-type MBD2 3’UTR by pre-miR-373 in HEK293 cells; C: Exogenous miR-373 down-regulates MBD2 protein in QBC₉₃₉; D: Epigenetic treatment of QBC₉₃₉ cells inhibites MBD2 protein following reactivation of miR-373 (Figure 3A). ^aP < 0.05, ^bP < 0.01 vs background. TSA: Trichostatin A; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.