Uncoupling protein 2 regulates glucagon-like peptide-1 secretion in L-cells

Figure 4 Knockdown of uncoupling protein-2 expression decreased oleic acid-induced secretion of glucagon-like peptide-1 in NCI-H716 cells. Up panel: The knock-down efficiency of small interfering RNA which targets UCP2 (siUCP2) in NCI-H716 cells. NCI-H716 cells were left untransfected (blank), were mock transfected (no siRNA), or were transfected with siUCP2. After 48 h, the mRNA levels of uncoupling protein-2 were determined as in Figure 4. Protein levels were determined as in Figure 4; Down panel: Effects of oleic acid on levels of secreted glucagon-like peptide-1 (GLP-1). NCI-H716 cells were transfected as described above and were then incubated with 8 mmol oleic acid for 2 h. GLP-1 concentrations were determined as in Figure 4. ^bP < 0.01 vs siRNA control.