Germline promoter hypermethylation of tumor suppressor genes in gastric cancer

Figure 2 Clonal bisulfite allelic sequencing of the MLH1 promoter in the peripheral blood from gastric cancer patient G46. A: Each horizontal line of circles represents an isolated allele. The numbering scheme is derived from the map shown in Figure 1A. White circles represent non-methylated CpG sites, and black circles indicate a methylated CpG. This subject displayed 10% methylated alleles at the 6th to 20th CpG sites, suggestive of mosaic allele-specific germline epimutation. Green, -93A; Red, -93G; B: Upper figure, sequencing of clone 5 from case G46 showing methylation at the 6th to 20th CpG sites. Lower figure, sequencing of G46 clone 7 revealing unmethylated CpGs except for the 9th and 10th CpG sites. The numbers above the sequences are derived from the map shown in Figure 1A, indicating the location of the CpG sites.