Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

Figure 2 Design of amplicons for in silico evaluation. Amplicons designed using the 6 universal primer sets as described in Table 3 were evaluated for theoretical forward and reverse reads. The schematic shows the relative position of each amplicon read and read direction, as well as which bases would be included in the sequence. The positional template is the Escherichia coli (E. coli) 16S rDNA gene with the approximate locations of the hypervariable regions labeled.