Fixation methods for electron microscopy of human and other liver

doi:10.3748/wjg.v16.i23.2851

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2010; 16(23): 2851-2866
Published online Jun 21, 2010. doi: 10.3748/wjg.v16.i23.2851

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Figure 11 SEM micrograph of the fenestrated endothelial lining of a human injection-fixed wedge biopsy. Note the grouping of fenestrae in sieve plates. Compare this figure with Figure 12, which shows an SEM micrograph of a mouse sinusoid at the same magnification. Fenestrae are apparently sensitive to fixation and are lost during immersion fixation. Their presence can therefore be used as a criterion of good fixation.

Citation: Wisse E, Braet F, Duimel H, Vreuls C, Koek G, Olde Damink SW, van den Broek MA, De Geest B, Dejong CH, Tateno C, Frederik P. Fixation methods for electron microscopy of human and other liver. World J Gastroenterol 2010; 16(23): 2851-2866
URL: https://www.wjgnet.com/1007-9327/full/v16/i23/2851.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i23.2851