Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Figure 2 Origin and morphology of LST-R1. A: The morphology of cultured cells under microscope at passage 5. The cells are polymorphic and the percentage of epithelial cells is more than 90% (× 10); B, C: Immunostaining for the epithelial markers ESA and CK20 in LST-R1 cells at passage 5. Cells were grown on chamber slides and stained for ESA (B) and CK20 (C) expression with a specific antibody; D: Under scanning electron microscope (× 1000), dense microvilli and tight junction were observed. Arrow indicates tight junction; E: cell observed under transmission electron microscope (× 6000). Arrow indicates nucleus; F: Representative karyotype of LST-R1 cell line. Aberrations were observed in the majority of LST-R1 cells. The karyotypic description is listed in Table 1.