Partial overlap of anti-mycobacterial, and anti-Saccharomyces cerevisiae mannan antibodies in Crohn’s disease

Figure 3 Association of anti-mycobacterial IgG with ASCA. CD patients were grouped according to their ASCA status and their anti-mycobacterial IgG were determined in comparison with healthy controls (n = 25). A: ASCA- (n = 42), extinction values at 450 nm below the cut-off as determined by routine diagnostics (3 standard deviations above the average of 10 negative control sera); ASCA+ (n = 48) extinction values above the cut-off. According to the legend of Figure 1, percentages of IgG-positive individuals per group were determined for each antigen. B: ASCA-positive CD patients were further grouped according to the levels of ASCA and compared to healthy controls and UC patients. ASCA+ (n = 28), A₄₅₀ clearly above, but less than double the cut-off value; ASCA++ (n = 10), A₄₅₀ 2 to 3 times higher than the cut-off value; ASCA+++ ( = 10), A₄₅₀ higher than 3 times the cut-off value. According to legends of Figures 1 and 2A, percentages of anti-LAM and anti-ManLAM IgG-positive individuals in each group were determined. Asterisks indicate significant differences between the groups. ^aP < 0.05, ^bP < 0.01.