Munc18/SNARE proteins’ regulation of exocytosis in guinea pig duodenal Brunner’s gland acini

Figure 5 SNARE localization after high carbachol (6 &mgr;mol/L) stimulation of Brunner’s gland acini. Confocal images of 6 &mgr;mol/L carbachol-stimulated Brunner’s gland acini labeled with anti-mucin antibody (in green) and double labeled with anti-Munc18c, Syntaxin-4 or SNAP-23 antibodies (in red), and an overlay (Merge) of the two with the DIC, which is also separately shown. Note the further reduced apical plasma membrane staining of Munc18c (vs Figure 4) and the absence of mucin staining in the cells. DIC images exhibit dilated lumens. Scale bars correspond to 10 &mgr;m.