Carbon liberated from CO-releasing molecules attenuates leukocyte infiltration in the small intestine of thermally injured mice

Figure 5 Effects of CORM-2 on NF-κB activation in the ileum tissue of thermally injured mice. Mice were challenged with thermal injury and treated with CORM-2 as described in Figure 1. Measurement of NF-κB activity was performed by EMSA with ³²P-labeled NF-κB probe and 5 μg nuclear extract from the ileum of sham, burn, burn + DMSO and burn+CORM-2 mice at 24 h after thermal injury. NF-κB activation in the ileum of thermally injured mice was markedly increased (lane 2), and this activity was inhibited by CORM-2 (lane 4). A representative experiment is shown in A, and quantitative results (average optical density) of three experiments are shown in B. ^aP < 0.05 vs sham-treated; ^cP < 0.05 vs burned.