High expression of hepatitis B virus based vector with reporter gene in hepatitis B virus infection system

Figure 2 Transduction of primary human hepatocytes (PHH) by the HBV-based vector. PHH were infected with rHBV-GFP, a recombinant HBV that carries a GFP gene under control of the UB promoter (described previously in Figure 1). A: Cell morphology of PHH on d 30; B: GFP expression on d 3 post-infection; C: GFP expression on d 6 post-infection; D: on d 12 post-infection; E: Transduction of PHH demonstrated by an overlay of the fluorescence; F: Phase contrast of the same field.