Gene expression analysis of primary normal human hepatocytes infected with human hepatitis B virus

Figure 1 Confirmation of HBV infection for PNHHs using PCR for cccDNA formation and RT-PCR for production of transcripts. From PNHHs infected with HBV, RNA and DNA were extracted using TRIzol reagent as described in Materials and Methods. As a negative control, mock-infected PNHHs were used. A: With the extracted DNA, cccDNA was confirmed by a PCR analysis as described in Materials and Methods; B: With the extracted RNA, a transcript of HBV was confirmed by RT-PCR analysis as described in Materials and Methods; C: With the extracted RNA, a tanscript of HBV was confirmed by real-time PCR as previously described[19].