Heterogeneity of the intrahepatic biliary epithelium

Figure 1 [Top] Isolation of small (A), approximately 8 μm diameter] and large (B), approximately 14 μm diameter] cholangiocytes from small and large ducts, respectively, from normal rats. Small and large cholangiocytes were purified by counterflow elutriation followed by immunoaffinity purification. Original magn., × 625. Reproduced with permission from Ref[12]. [Bottom] Isolation of small (C) and large (D) IBDU from normal rat liver. Small (< 15 μm in diameter) and large (> 15 μm in diameter) IBDU were pruned off from large ducts by a nitrogen pulsed dye laser and subsequently separated (D) by picking up IBDU with a micromanipulator micropipet. Original magnification × 2000. Reproduced with permission from Ref 13.