An efficient method of sorting liver stem cells by using immuno-magnetic microbeads

Figure 2 Flow cytometric analysis of fetal mouse liver cells before and after immuno-magnetic sorting. A: Two-color FACS analysis of ED 13 mouse fetal liver cells by FACSCalibur. Cells were stained as described in Figure 1A. B: Histograms for the immuno-magnetically sorted (CD45/TER119)^– cells labeled with phycoerythrin-streptavidin (blue) and the total cell populations before sorting (white). C: FACS analysis of ED 13 mouse fetal liver cells stained with the biotinylated anti-c-Kit mAb. D: Analysis of the immuno-magnetically sorted c-Kit^– cells labeled with phycoerythrin-streptavidin (blue). The histogram of Figure 2B is also shown (white).