Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants

Figure 5 MKN45 cells were preincubated for 60 min with anti CD44v4, anti CD44v5 or anti CD44v7 monoclonal antibodies at 1:80 dilution. MKN45 cells were then added to HUVEC monolayer to evaluate the adhesion capacity (right part of Figure 5). In a second set of experiments, HUVEC were treated with hyaluronidase for 60 min (1 000, 2 500, or 5 000 U/mL), before MKN45 cells were added (left part of Figure 5). Non treated HUVEC served as the 100% control. Mean±SD of n = 6 experiments.