Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 11 Effect of t10c12 on HRG-β-induced ERK-1/2 activation in HT-29 cells. Similar protein samples as those in Figure 8 were analyzed by immunoblotting with p-ERK-1/2 or whole ERK-1/2 antibodies. A: Photographs of chemiluminescent detection of the blots, which were representative of three independent experiments, are shown; B: Quantitative analysis of immunoblots. The relative fold change in p-ERK-1/2 to its own ERK-1/2 control band on Western blots was quantitated by densitometric analysis. Each bar represents the mean±SE (n = 3).