Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 9 Effect of t10c12 and/or HRG-β on ErbB3-associated PI3K activity. Cells were treated, and lysates were prepared and immunoprecipitated with an anti-ErbB3 antibody as described in Figure 8. The immune complexes were incubated with phosphatidylinositol and [g-³²P]ATP. Phosphatidylinositol 3-phosphate (PIP) generated by immunoprecipitated PI3K was separated by thin-layer chromatography (TLC). A: An autoradiograph of the TLC plate, which was representative of three independent experiments, is shown; B: Relative fold changes in PIP. The radioactive PIP signals were quantitated by densitometry. Each bar represents the mean±SE (n = 3).