Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

Figure 3 Analyses of refolded A2-GIL (A) and A2-NLV (B) monomers after purification and biotinylation with SDS-PAGE (150 g/L). In (A) lane 1: protein MW marker; lane 2: solubilized β₂m; lane 3: solubilized A2-BSP; lane 4: refolded A2-GIL monomer; lane 5: biotinylated A2-GIL monomer; lane 6: peak I (β₂m) (Figure 4); lane 7: peak II (purified A2-GIL). In (B) lane 1: protein MW marker; lane 2: refolded A2-NLV monomer; lane 3: biotinylated A2-NLV monomer; lane 4: peak I (β₂m); lane 5: peak II (purified A2-NLV). HC: heavy chain.