Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn’s disease and contributes to accelerated epithelial wound healing in vitro

Figure 2 Differential gene expression of Eph-RTKs and ephrins in the mucosa of IBD patients (TaqMan^® RT-PCR validation of Affymetrix^® cDNA array results[17]). Individual RNAs from perilesional total mucosa biopsies (ileum terminale and colon) of 4 control subjects, 4 MC patients and 4 CU patients were analyzed. Real-time RT-PCR confirmed significant up-regulation of ephrin-B2 in MC patients (ileum and colon) and CU (colon). Differential expression of EphB4, as suggested by the Affymetrix^® array data, was not significant. The relative expression values were calculated according to the standard curve method of the TaqMan^® protocol (Applied Biosystems, Darmstadt, Germany) by normalization with a housekeeper gene (18S rRNA) and are therefore unitless (for details, see[15]). ^aP < 0.05 vs normal.