Enhancement of humoral immune responses to HBsAg by heat shock protein gp96 and its N-terminal fragment in mice

Figure 4 T-cell response of splenocytes of immunized Balb/c mice. Mice immunized with PBS, HBsAg plus heated NTF, HBsAg plus NTF or gp96 (the same groups as groups 5, 6, and 8 respectively in Figure 3) were killed on d 63 and the splenocytes were collected for ELISPOT assay with 10⁵ cells per well. The number of SFCs was calculated. The photograph of the representative wells from different groups is shown below the statistics.