Expression of von Hippel-Lindau tumor suppressor and tumor-associated carbonic anhydrases IX and XII in normal and neoplastic colorectal mucosa

Figure 1 Schematic drawing of pVHL domain composition. Epitope region for the monoclonal antibodies VHL06 and VHL40 used in immunohistochemistry is shown below the scheme[30]. Positions of primers (a = antisense, s = sense) used for the amplification of two overlapping RT-PCR products are indicated above the exon map. Involvement of HIF-α-binding β-domain and elongin C-binding α-domain of pVHL in the negative regulation of CA XII and CA IX is illustrated according to data described by Ivanov et al[6].