Expansion and activation of natural killer cells from PBMC for immunotherapy of hepatocellular carcinoma

Figure 2 Killing by T cell- and NK-enriched lymphocytes. Lym-phocytes derived from PBMC of the Subject-1 (taken from Experiment-1 of Table 1) were used in this 24-h crystal violet staining with various E/T ratios. Eight columns on left side are the results from assays of lymphocytes grown on irradiated TKB-17RGB cells (MHC class I positive). Major lymphocyte population was CD3+CD56- (T cell-enriched, see Table 1). For the killing assay, fresh TKB-17RGB (left-end 4 columns) or cells (mid-left 4 columns) were submitted as the target. Right side 8 columns are the results from assays of lymphocytes grown on irradiated HFWT cells (MHC class I negative). Major lympho-cyte population was CD3-CD56+ (NK-enriched, see Table 1). For the killing assay, fresh TKB-17RGB (mid-right 4 columns) or HFWT cells (right-end 4 columns) were used as the target. Each column represents the mean value of triplicate measurements.