Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration

Figure 5 Agarose gel electrophoresis of PCR products. The PCR steps were depicted in Figure 1. Template in lanes 1, 2 and 5 is the mixture from gene fragments A and B; while tem-plate in lanes 3 and 4 is the BPCR product (lane 5). Lane 1, primer Sg1P5/RAC3; lane 2, primer POP51/POP31; lane 3, POP-PCR with primer POP51+POP52/POP31+POP32; lane 4, general PCR of the ORF of D106 with primer pair Sg1P5/DEN3; lane 5, BPCR of full-length D106 with primer Sg1P5/RAC3; M, molecular marker.