Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 7, 2016; 22(41): 9127-9140
Published online Nov 7, 2016. doi: 10.3748/wjg.v22.i41.9127
Esophagogastric anastomosis in rats: Improved healing by BPC 157 and L-arginine, aggravated by L-NAME
Zeljko Djakovic, Ivka Djakovic, Vedran Cesarec, Goran Madzarac, Tomislav Becejac, Goran Zukanovic, Domagoj Drmic, Lovorka Batelja, Anita Zenko Sever, Danijela Kolenc, Alen Pajtak, Nikica Knez, Mladen Japjec, Kresimir Luetic, Dinko Stancic-Rokotov, Sven Seiwerth, Predrag Sikiric
Zeljko Djakovic, Ivka Djakovic, Vedran Cesarec, Goran Madzarac, Tomislav Becejac, Goran Zukanovic, Domagoj Drmic, Lovorka Batelja, Anita Zenko Sever, Danijela Kolenc, Alen Pajtak, Nikica Knez, Mladen Japjec, Kresimir Luetic, Dinko Stancic-Rokotov, Sven Seiwerth, Predrag Sikiric, Departments of Pharmacology and Pathology Medical Faculty University of Zagreb, 10000 Zagreb, Croatia
Author contributions: Djakovic Z, Djakovic I, Drmic D, Batelja L, Luetic K, Stancic-Rokotov D, Seiwerth S and Sikiric P designed the research; Djakovic Z, Djakovic I, Cesarec V, Madzarac G, Becejac T, Zukanovic G, Drmic D, Batelja L, Zenko Sever A, Kolenc D, Pajtak A, Knez N, Japjec M and Luetic K performed the research; Stancic-Rokotov D, Seiwerth S and Sikiric P contributed reagents and analytic tools; Djakovic Z, Djakovic I, Becejac T, Drmic D, Batelja L, Zenko Sever A, Luetic K, Stancic-Rokotov D, Seiwerth S and Sikiric P analyzed the data; Djakovic Z, Djakovic I, Drmic D, Batelja L, Stancic-Rokotov D, Seiwerth S and Sikiric P wrote the paper.
Supported by Ministry of Science, Education and Sports, Republic of Croatia, No. 108-1083570-3635.
Institutional review board statement: The study was reviewed and approved by the Department of Veterinary, Ministry of Agriculture, Republic of Croatia, No: UP/I 322-01/07-01/210.
Conflict-of-interest statement: The authors state that they have no conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Predrag Sikiric, MD, PhD, Professor, Departments of Pharmacology and Pathology Medical Faculty University of Zagreb, Salata 11, POB 916, 10000 Zagreb, Croatia. sikiric@mef.hr
Telephone: +385-1-4566833 Fax: +385-1-4920050
Received: July 1, 2016
Peer-review started: July 2, 2016
First decision: August 8, 2016
Revised: August 28, 2016
Accepted: September 28, 2016
Article in press: September 28, 2016
Published online: November 7, 2016
Core Tip

Core tip: In rats underwent esophagogastric anastomosis, BPC 157 (given intraperitoneally or in drinking water) fully counteracted an otherwise serious disease course since very beginning (i.e., with BPC 157 bath blood vessels remained present at the gastric surface after anastomosis creation) and eliminated mortality. Additionally, BPC 157 treatment, along with L-NAME, nullified any effect of L-NAME that otherwise intensified the regular course. Consistently, with worsening (with L-NAME administration) and amelioration (with L-arginine), either L-arginine-amelioration prevails (i.e., esophageal and gastric lesions are attenuated) or they counteract each other (L-NAME + L-arginine), an effect which is further reversed toward a marked beneficial effect with the addition of BPC 157 (L-NAME + L-arginine + BPC 157).