Schreiber D, Jost V, Bischof M, Seebach K, Lammers WJ, Douglas R, Schäfer KH. Motility patterns of ex vivo intestine segments depend on perfusion mode. World J Gastroenterol 2014; 20(48): 18216-18227 [PMID: 25561789 DOI: 10.3748/wjg.v20.i48.18216]
Corresponding Author of This Article
Karl-Herbert Schäfer, MD, Enteric Nervous System Group, University of Applied Sciences Kaiserslautern, 1 Amerikastrasse, Zweibrücken 66482, Germany. karl-herbert.schaefer@hs-kl.de
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Original Article
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World J Gastroenterol. Dec 28, 2014; 20(48): 18216-18227 Published online Dec 28, 2014. doi: 10.3748/wjg.v20.i48.18216
Motility patterns of ex vivo intestine segments depend on perfusion mode
Dominik Schreiber, Viktor Jost, Michael Bischof, Kristina Seebach, Wim JEP Lammers, Rees Douglas, Karl-Herbert Schäfer
Dominik Schreiber, Viktor Jost, Michael Bischof, Kristina Seebach, Karl-Herbert Schäfer, Enteric Nervous System Group, University of Applied Sciences Kaiserslautern, Zweibrücken 66482, Germany
Wim JEP Lammers, College of Medicine and Health Sciences, United Arab Emirates University, Al-Ain 17666, United Arab Emirates
Rees Douglas, Aqix Limited, Imperial College Incubator, London SW7 2AZ, United Kingdom
Author contributions: Schreiber D wrote the manuscript and interpreted data; Jost V, Bischof M and Seebach K performed the research and analyzed data; Lammers WJEP contributed to the design of the study and provided analytic tools; Douglas R provided reagents used in this study; Schäfer KH designed the study, provided financial support and corrected the manuscript.
Correspondence to: Karl-Herbert Schäfer, MD, Enteric Nervous System Group, University of Applied Sciences Kaiserslautern, 1 Amerikastrasse, Zweibrücken 66482, Germany. karl-herbert.schaefer@hs-kl.de
Telephone: +49-631-37245418 Fax: +49-631-37245308
Received: April 18, 2014 Revised: June 8, 2014 Accepted: July 11, 2014 Published online: December 28, 2014
Core Tip
Core tip: Perfused intestinal segments can be used as a pharmacological model in order to test drugs acting on motility and gastrointestinal physiology. Different motility patterns can be distinguished using visualization and appropriate software tools. In order to use intestinal segments for pharmacological testing it is important to know which factors affect intestinal motility. Motility measurement and characterization of motility pattern in the ex vivo system is much easier compared to in vivo measurements. The main disadvantage of the ex vivo system is its limited stability. Therefore longer ex vivo times are desirable. In this study we could demonstrate effects of different perfusion media on tissue viability and visualize motility in response to perfusion conditions.
