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©2014 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2014; 20(25): 8130-8138
Published online Jul 7, 2014. doi: 10.3748/wjg.v20.i25.8130
Published online Jul 7, 2014. doi: 10.3748/wjg.v20.i25.8130
Phasic study of intestinal homeostasis disruption in experimental intestinal obstruction
Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China
Chang-Lin Zou, Graduate School of Tianjin Medical University, Tianjin 300100, China
Dong-Hua Li, Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China
Nai-Qiang Cui, Department of Hepatopancreatobiliary Surgery, Nankai Hospital, Tianjin 300100, China
Author contributions: Yu XY and Zou CL contributed equally to this study and performed the majority of the experiments; Zhou ZL, Shan T and Li DH participated in the performance of experiments; Yu XY and Cui NQ designed the study and wrote the manuscript.
Supported by National Program on Key Basic Research Project, No. 2009CB522703
Correspondence to: Xiang-Yang Yu, MD, Department of Gastrointestinal Surgery, Nankai Hospital, Nankai District, No. 6 Changjiang Rd, Tianjin 300100, China. yxynankai@126.com
Telephone: +86-22-27435860 Fax: +86-22-27435860
Received: September 3, 2013
Revised: February 21, 2014
Accepted: April 1, 2014
Published online: July 7, 2014
Revised: February 21, 2014
Accepted: April 1, 2014
Published online: July 7, 2014
Core Tip
Core tip: A controllable rabbit model of intestinal obstruction was established. This model demonstrated that intestinal obstruction (1) induced intestinal epithelial cell damage and reduction; (2) disrupted the balance of intestinal microorganisms by abnormal proliferation of pathogenic bacteria; and (3) disrupted the intestinal immune system, observed as a decrease in the number and area of Peyer’s patches, and alteration of CD4+ T cell number and CD4/CD8+ T cell ratio. Furthermore, the levels of ornithine decarboxylase activity and citrulline and claudin 1 expression could serve as indicators of intestinal epithelial cell damage.