Lu LG, Zeng MD, Li JQ, Hua J, Fan JG, Fan ZP, Qiu DK. Effect of lipid on proliferation and activation of rat hepatic stellate cells (I). World J Gastroenterol 1998; 4(6): 497-499 [PMID: 11819353 DOI: 10.3748/wjg.v4.i6.497]
Corresponding Author of This Article
Dr. Lun-Gen Lu, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, 145 Shandong Zhonglu, Shanghai 200001, China.
Article-Type of This Article
Original Articles
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Lun-Gen Lu, Min-De Zeng, Ji-Qiang Li, Jing Hua, Jian-Gao Fan, Zhu-Ping Fan, De-Kai Qiu, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Lun-Gen Lu, M.D., male, born on November 9, 1965 in Yangzhou City, Jiangsu Province, specializing in the experimental and clinical study of digestive diseases, and having more than 20 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Lun-Gen Lu, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, 145 Shandong Zhonglu, Shanghai 200001, China.
Received: September 7, 1998 Revised: November 2, 1998 Accepted: December 5, 1998 Published online: December 15, 1998
Abstract
AIM: To study the effect of lipid (triglyceride and very low-density lipoprotein, VLDL) on proliferation and activation of rat hepatic stellate cells (HSC).
METHODS: HSC were isolated and cultured from liver of Wistar rats by in situperfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. HSC proliferation was examined with MTT colorimetric assay.
RESULT: Triglyceride of 12.5 mg/L had a promoting effect on proliferation of HSC (P < 0.05), 25, 50, 100 and 200 mg/L had no effects (P > 0.05), but 400 mg/L had an inhibiting effect (P < 0.01). VLDL of 6.25 and 12.5 mg/L had no effect on proliferation of HSC (P > 0.05), but increased concentration of VLDL could promote the HSC proliferation (P < 0.05).
CONCLUSION: Lipid had an effect on proliferation of HSC. Triglyceride and VLDL may promote HSC proliferation and may be associated with fatty liver and hepatic fibrogenesis.
