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©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 1998; 4(2): 106-108
Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.106
Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.106
Detection of hepatoma cells in peripheral blood of HCC patients by nested RT-PCR
Yu-Hui Liu, Rou-Li Zhou, Department of Cell Biology, Beijing Medical University, Beijing 100083, China
Jing-An Rui, Institute of Hepatocarcinoma Research, General Hospital, Post and Telecommunication Ministry, Beijing 100032, China
Yu-Hui Liu, graduated from Beijing Medical University in 1995, now is working in the Department of Cell Biology, Beijing Medical University.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Rou-Li Zhou, Department of Cell Biology, Beijing Medical University, Beijing 100083, China. rlzhou @mail.bjmu.edu.cn.
Telephone: +86·10·62091476 Fax: +86·10·62358270
Received: October 28, 1997
Revised: December 1, 1997
Accepted: January 10, 1998
Published online: April 15, 1998
Revised: December 1, 1997
Accepted: January 10, 1998
Published online: April 15, 1998
Abstract
AIM: To offer a more simple method with a high sensitivity and specificity for detection of hepatoma cells in peripheral blood of the patients with HCC.
METHODS: Improved nested RT-PCR method was used to detect the expression of AFP mRNA in nuclear cells separated from peripheral venous blood.
RESULTS: AFP mRNA contained in ten hepatoma cells was detected from 2 mL peripheral blood.
CONCLUSION: The improved nested RT-PCR assay for AFP mRNA expressed in cancer cells in peripheral blood might be a valuable method for clinical diagnosis of HCC.
Keywords: liver neoplasms, carcinoma, hepatocellular, RNA, messenger/blood, alpha-feto-proteins polymerase chain reaction, neoplasm metastasis