Basic Research
Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 7, 2007; 13(45): 5989-5994
Published online Dec 7, 2007. doi: 10.3748/wjg.v13.i45.5989
Protective effects of medical ozone combined with traditional Chinese medicine against chemically-induced hepatic injury in dogs
Li-Jie Li, Yun-Gao Yang, Zhi-Ling Zhang, Sui-Feng Nie, Ze Li, Feng Li, He-Yu Hua, Yan-Jun Hu, Hong-Shuan Zhang, Ya-Bing Guo
Li-Jie Li, Zhi-Ling Zhang, Sui-Feng Nie, Ze Li, Feng Li, No. 425 Central Hospital of PLA, Sanya 572008, Hainan Province, China
Yun-Gao Yang, He-Yu Hua, Hong-Shuan Zhang, Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Yan-Jun Hu, Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Ya-Bing Guo, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Superintendent Foundation of Nanfang Hospital in Guangzhou, Guangdong Province, No. 20050012, the Foundation of Health Bureau of Guangdong Province, No. 2006388, and the Foundation for Studies of the Science and Technology Bureau of Shenzhen, Guangdong Province, No. 200204176
Correspondence to: Associate Professor Ya-Bing Guo, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. yabing@fimmu.com
Telephone: +86-20-61641947 Fax: +86-20-87719653
Received: March 30, 2007
Revised: July 14, 2007
Accepted: September 26, 2007
Published online: December 7, 2007
Abstract

AIM: To investigate the protective effect of medical ozone (O3) combined with Traditional Chinese Medicine (TCM) Yigan Fuzheng Paidu Capsules (YC) against carbon tetrachloride (CCl4)-induced hepatic injury in dogs.

METHODS: Thirty healthy dogs were divided randomly into five groups (n = 6 in each group), namely control, oleanolic acid tablet (OAT), O3, YC and O3 + YC, given either no particular pre-treatment, oral OAT, medical ozone rectal insulfflation every other day, oral YC, or oral YC plus medical ozone rectal insulfflation every other day, respectively, for 30 consecutive days. After pre-treatment, acute hepatic injury was induced in all dogs with a single-dose intraperitoneal injection of CCl4. General condition and survival time were recorded. The biochemical and hematological indexes of alanine aminotransferase (ALT), aspartate aminotransferase/alanine aminotransferase (AST/ALT), serum total bilirubin (TBIL), prothrombin time (PT), blood ammonia (AMMO), and blood urea nitrogen (BUN) were measured after CCl4 injection. Hepatic pathological changes were also observed.

RESULTS: Compared to the other four groups, the changes of group O3 + YC dogs’ general conditions (motoricity, mental state, eating, urination and defecation) could be better controlled. In group O3 + YC the survival rates were higher (P < 0.05 vs group control). AST/ALT values were kept within a normal level in group O3 + YC. Hepatic histopathology showed that hepatic injury in group O3 + YC was less serious than those in the other four groups.

CONCLUSION: Medical ozone combined with TCM YC could exert a protective effect on acute liver injury induced by CCl4.

Keywords: Carbon tetrachloride, Ozone, Traditional Chinese Medicine