Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes

doi:10.3748/wjg.v12.i3.453

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Jan 21, 2006 (publication date) through May 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 21, 2006; 12(3): 453-456
Published online Jan 21, 2006. doi: 10.3748/wjg.v12.i3.453

Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes

Jun Li, Zhi-Hua Feng, Guang-Yu Li, Dan-Lei Mou, Qing-He Nie

Jun Li, Zhi-Hua Feng, Guang-Yu Li, Dan-Lei Mou, Qing-He Nie, The Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China

Supported by the National Natural Science Foundation of China, No. 30170822

Correspondence to: Professor Qing-He Nie, The Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China. fengzh@fmmu.edu.cn

Telephone: +86-29-83377165

Received: July 19, 2005
Revised: July 20, 2005
Accepted: July 22, 2005
Published online: January 21, 2006

Abstract

AIM: To generate dendritic cells (DCs) from human peripheral blood and to detect the expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN; CD209) for the further study of DC-SIGN in hepatitis C virus (HCV) transmission.

METHODS: Peripheral blood monocytes were isolated from blood of healthy individuals by Ficoll£Hypaque sedimentation and cultured in complete medium containing rhGM-CSF and rhIL-4. Cells were cultured for seven days, with cytokine addition every two days to obtain immature DCs. Characteristics of the cultured cells were observed under light and scanning microscope, and the expression of DC-SIGN was detected by immunofluorescence staining.

RESULTS: After seven-day culture, a large number of cells with typical characteristics of DCs appeared. Their characteristics were observed under light and scanning electron microscope. These cells had a variety of cell shapes such as those of bipolar elongate cells, elaborate stellate cells and DCs. DC-SIGN was detected by immunofluorescence staining and its expression level on cultivated dendritic cells was high.

CONCLUSION: DCs with a high expression of DC-SIGN can be generated from human peripheral blood monocytes in complete medium containing rhGM-CSF and rhIL-4.

Keywords: Expression of DC-SIGN, Dentric cells, Peripheral blood monocytes.