Colorectal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2005; 11(25): 3871-3876
Published online Jul 7, 2005. doi: 10.3748/wjg.v11.i25.3871
Urinary nucleosides as biological markers for patients with colorectal cancer
Yu-Fang Zheng, Jun Yang, Xin-Jie Zhao, Bo Feng, Hong-Wei Kong, Ying-Jie Chen, Shen Lv, Min-Hua Zheng, Guo-Wang Xu
Yu-Fang Zheng, Jun Yang, Xin-Jie Zhao, Hong-Wei Kong, Shen Lv, Guo-Wang Xu, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China
Bo Feng, Min-Hua Zheng, Department of Surgical, the Ruijin Affiliated Hospital of Shanghai Second Medical University, Shanghai 200025, China
Ying-Jie Chen, Shen Lv, Center of Experiment, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Supported by the High-tech R and D Plan, No. 2003AA223061 and the Sociality Commonweal Project of State Ministry of Science and Technology of China, the Knowledge Innovation Program of the Chinese Academy of Sciences, No. K2003A16 and Liaoning Province Foundation of Science and Technology
Correspondence to: Professor Dr. Guo-Wang Xu, National Chromatographic R and A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China. dicp402@mail.dlptt.ln.cn
Telephone: +86-411-84379530 Fax: +86-411-84379559
Received: October 2, 2004
Revised: December 1, 2004
Accepted: December 3, 2004
Published online: July 7, 2005
Abstract

AIM: Fourteen urinary nucleosides, primary degradation products of tRNA, were evaluated to know the potential as biological markers for patients with colorectal cancer.

METHODS: The concentrations of 14 kinds of urinary nucleosides from 52 patients with colorectal cancer, 10 patients with intestinal villous adenoma and 60 healthy adults were determined by column switching high performance liquid chromatography method.

RESULTS: The mean levels of 12 kinds of urinary nucleosides (except uridine and guanosine) in the patients with colorectal cancer were significantly higher than those in patients with intestinal villous adenoma or the healthy adults. Using the levels of 14 kinds of urinary nucleosides as the data vectors for principal component analysis, 71% (37/52) patients with colorectal cancer were correctly classified from healthy adults, in which the identification rate was much higher than that of CEA method (29%). Only 10% (1/10) of patients with intestinal villous adenoma were indistinguishable from patients with colorectal cancer. The levels of m1G, Pseu and m1A were positively related with tumor size and Duke’s stages of colorectal cancer. When monitoring the changes in urinary nucleoside concentrations of patients with colorectal cancer associated with surgery, it was found that the overall correlations with clinical assessment were 84% (27/32) and 91% (10/11) in response group and progressive group, respectively.

CONCLUSION: These findings indicate that urinary nucleosides determined by column switching high performance liquid chromatography method may be useful as biological markers for colorectal cancer.

Keywords: Nucleosides, Biological markers, Colorectal cancer, High performance liquid chromatography