Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder

Figure 5 Magnetic resonance imaging, microcholangiography and histomorphology of a rat on day 60 after surgically induced cholelithiasis. A: Coronal T2-w MRI shows a hypointense gallstone located in a hyperintense VGB and distal nondilated CBD as a hyperintense line with L and S denoting the liver and stomach; B: Digital microcholangiography displays both the hyperdense VGB and distal nondilated CBD bridged in-between by an pancreatic ductule with P denoting the pancreas; C: Macroscopy views the VGB with L denoting the liver, note the distal nondilated and barium-filled CBD imbedded in the pancreas (P) and a branching ductule bridging the ligation between the proximal and distal CBD, suggesting resumed bile flow, asterisk indicates where the needle hole was closed by suture ligation; D: Photomicrograph of hematoxylin and eosin stained slide of the distal CBD near the ligature (dashed square on B and C) demonstrates multiple proliferated glandular ductules (arrows) that contribute to the eventual bypass of the biliary obstruction between the VGB and distal CBD (original magnification × 100). MRI: Magnetic resonance imaging; CBD: Common bile duct; VGB: Virtual gallbladder; PD: Pancreatic duct.