C5b-9 does not mediate tubulointerstitial injury in experimental acute glomerular disease characterized by selective proteinuria

Figure 1 The tubulointerstitial deposition of C5b-9 is increased in C6⁺ rats with protein-overload nephropathy. Representative sections are shown. A: Animal injected with saline (control); B: C6⁺ animal with PON on day 4; C: C6⁺ animal with PON on day 8; D: C6- animal with PON on day 8 (× 400 magnification). Predominant basolateral deposition of C5b-9 was present in PON. PON: Protein-overload nephropathy.

Figure 2 Cortical tubulointerstitial injury in control and protein-overload nephropathy groups on day 8. Representative renal cortical sections (magnification × 400) show immunostaining for BrdU, periodic acid-schiff and ED-1.

Figure 3 The renal cortical expression of proliferating cell nuclear antigen is increased in protein-overload nephropathy. Representative sections (× 200 magnification) are shown. PCNA positive TEC nuclei are labeled purple (Nickel-enhanced DAB-positive, arrows). Brush border are labeled with anti-Fx1A (brown) and counterstained with periodic acid-schiff. PCNA: Proliferating cell nuclear antigen; TEC: Tubular epithelial cells.