Basic Study
Copyright ©The Author(s) 2024.
World J Nephrol. Sep 25, 2024; 13(3): 95627
Published online Sep 25, 2024. doi: 10.5527/wjn.v13.i3.95627
Figure 1
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Figure 1 Experimental protocol of omega-3 administration.
Figure 2
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Figure 2 Representative micrographs of the renal slices (hematoxylin and eosin) from bile duct ligated and sham-operated (control) rats. A: Control group; B: Bile-duct-ligated group.
Figure 3
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Figure 3 Effect of omega-3 on the generation of free radicals and lipoperoxidation in the kidneys of cirrhotic rats. A: In vivo effect of omega-3 on flow cytometry detection of reactive oxygen species in renal homogenates. Mean data for dichlorofluorescein fluorescence; B: Effect of omega-3 on lipoperoxidation measured by thiobarbituric acid reactive substances in the kidney of rats. Values represent the mean ± SD of n = 6 animals per group. aP ≤ 0.001 vs control group; cP ≤ 0.001 vs BDL with BDL plus omega group. DCF: Dichlorofluorescein; TBARS: Thiobarbituric acid reactive substances; BDL: Bile duct ligation.
Figure 4
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Figure 4 In vivo effect of omega-3 on mitochondrial membrane potential in the kidney of rats. Values represent the mean ± SD of n = 6 animals per group. aP ≤ 0.001 versus control group, bP ≤ 0.01 versus BDL with BDL plus omega group. BDL: Bile duct ligation.
Figure 5
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Figure 5 Effect of omega-3 on the activity of antioxidant enzymes measured in the kidneys of cirrhotic rats. A: In vivo effect of omega-3 on superoxide dismutase activities in the kidneys of rats. B: In vivo effect of omega-3 on catalase activities in the kidneys of rats. Values represent the mean ± SD of n = 6 animals per group. aP ≤ 0.001 versus control group, bP ≤ 0.01 and cP ≤ 0.001 versus BDL with BDL plus omega group. BDL: Bile duct ligation; SOD: Superoxide dismutase; CAT: Catalase.
Figure 6
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Figure 6 In vivo effects of omega-3 on nitric oxide production. Values represent the mean ± SD of n = 6 animals per group. aP ≤ 0.001 versus control group, cP ≤ 0.001 versus BDL with BDL plus omega group. BDL: Bile duct ligation; NO: Nitric oxide.
Figure 7
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Figure 7 Effect of omega-3 on DNA damage and cell survival in the kidney of cirrhotic rats. A: In vivo effect of omega-3 on DNA damage in the kidneys of rats; B: Effect of omega-3 on cell viability determined by trypan blue dye-exclusion assay; C: Effect of omega-3 on caspase 9 cleaved. Values represent the mean ± SD of n = 6 animals per group. aP ≤ 0.001 versus control group, bP ≤ 0.01 and cP ≤ 0.001 versus BDL with BDL plus omega group. BDL: Bile duct ligation.