Substrate specificity of avian influenza H5N1 neuraminidase

doi:10.5501/wjv.v3.i4.30

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Nov 12, 2014 (publication date) through Jul 9, 2024

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World Journal of Virology

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2220-3249

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Virology. Nov 12, 2014; 3(4): 30-36
Published online Nov 12, 2014. doi: 10.5501/wjv.v3.i4.30

Table 1 Virus strains and sources

Virus	Subtype	Passage	Source
A/Thailand/KAN-1A/2004	H5N1	MDCK8	Human
A/Thailand/676/2005	H5N1	MDCK8	Human
A/Thailand/3(SP-83)/2004	H5N1	MDCK8	Human
A/Openbill stork/Thailand/VSMU-4-NSA/2004	H5N1	MDCK4	Avian
A/Openbill stork/Thailand/VSMU-5-NSA/2004	H5N1	MDCK4	Avian
A/Chicken/Bangkok/VS-MU-1/2006	H5N1	MDCK4	Avian
A/Chicken/Thailand/BF2037/2007	H5N1	MDCK4	Avian
A/Openbill stork/Thailand (Nakhonsawon)/VSMU-32/2005	H5N1	MDCK4	Avian

MDCK: Madin-Darby canine kidney.

Table 2 Neuraminidase activity measured by high-performance liquid chromatography analysis

Linkage type	Siaαx Lac-PA	DiSiaαx BI-PA		4Siaαx Tetra-PA
	Digested	One sialic digested	Two sialic digested	Completely digested
α2,3	100	3.5%	96.5%	100%
α2,6	0	0	0	ND

ND: Not done.

Citation: Onsirisakul N, Nakakita SI, Boonarkart C, Kongchanagul A, Suptawiwat O, Puthavathana P, Chaichuen K, Kittiniyom K, Suzuki Y, Auewarakul P. Substrate specificity of avian influenza H5N1 neuraminidase. World J Virology 2014; 3(4): 30-36
URL: https://www.wjgnet.com/2220-3249/full/v3/i4/30.htm
DOI: https://dx.doi.org/10.5501/wjv.v3.i4.30