Original Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Virol. Aug 12, 2013; 2(3): 123-135
Published online Aug 12, 2013. doi: 10.5501/wjv.v2.i3.123
Table 1 Primers used to amplify hepatitis delta virus cDNA fragments
HDV cDNA fragmentsForward primer 5'3'Reverse primer 5'3'Fragment length (bp)Genomelocation(Sense)Antigenomelocation(Antisense)
A1CGCATCGATACTCCCTGCAGATTGGGGACGCATCGATATTCACCGACAAGGAGAGGC243360-6021078-1320
A2CGCATCGATGCCTCTCCTTGTCGGTGAATCGCATCGATGAGACCTCCGGAAGACAAAGA204176-3791301-1504
A3CGCATCGATTTCCGGAGGTCTCTCTCGAGTCGCATCGATTCTCCTCGCTCGGAACTTG2141654-679/1881492-1679/26
A4CGCATCGATTTCCTCGGTCAACCTCCTGACGCATCGATATAAGGATGGAGAGGGGGCT224266-4891191-1414
A5CGCATCGATCCCCTCTCCATCCTTATCCTCGCATCGATAGGGAGAGAAGAGATCCTCGA172114-2851395-1566
A6CGCATCGATCGAATGGGACCCACAAATCTCGCATCGATTCCCCAATCTGCAGGGAGT337584-920760-1096
A7CGCATCGATCCCAATCCCAGATCTGGAGACGCATCGATTTTGCTTTCCTCCTCGCTTC2041263-1466214-417
A8CGCATCGATAAAGAAAGCAACGGGGCTAGCGCATCGATGGGAGTCGGAATCGAGCAT1991067-1265415-613
A9CGCATCGATATGCTCGATTCCGACTCCCCGCATCGATCCTAGAGAGATTTGTGGGTCCC191895-1085595-785
A10CGCATCGATCAAGTTCCGAGCGAGGAGACCGCATCGATTCTCCAGATCTGGGATTGGG2271446-16728-234
Table 2 Primers used to amplify truncated forms of the A7AS agRNA sequence
HDV cDNA fragmentsForward primer 5'3'Reverse primer 5'3'Fragment length (bp)
269-417CGCATCGATGGGAGGAATCCACTCGGAGACGCATCGATTTTGCTTTCCTCCTCGCTTC149
214-379CGCATCGATCCCAATCCCAGATCTGGAGACGCATCGATGCATCTCCTCCTATCGCTATGG166
214-403CGCATCGATCCCAATCCCAGATCTGGAGACGCATCGATGCTTCGGTCTCCCCCTACTC190
244-417CGCATCGATCCCGAAGGGTTGAGTAGCACCGCATCGATTTTGCTTTCCTCCTCGCTTC174
244-403CGCATCGATCCCGAAGGGTTGAGTAGCACCGCATCGATGCTTCGGTCTCCCCCTACTC160
314-417CGCATCGATACCCCTTCAGCGAACAAGAGCGCATCGATTTTGCTTTCCTCCTCGCTTC104
214-322CGCATCGATCCCAATCCCAGATCTGGAGACGCATCGATTGAAGGGGTCCTCGGAGGT109
269-379CGCATCGATGGGAGGAATCCACTCGGAGACGCATCGATGCATCTCCTCCTATCGCTATGG111