Evaluation of virus-specific cellular immune response in transplant patients

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Dec 12, 2012 (publication date) through Aug 24, 2024

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World Journal of Virology

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2220-3249

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Virol. Dec 12, 2012; 1(6): 150-153
Published online Dec 12, 2012. doi: 10.5501/wjv.v1.i6.150

Table 1 Methods for evaluating cellular immune response

Method	Advantages	Limitations
Intracellular cytokine staining	Low volume blood	Flow cytometer required
	Rapid	Not standardized
	Knowledge of HLA not required
	Identification of CD4+ and CD8+
MHC multimer staining	Low volume blood	Flow cytometer required
	Rapid	Not standardized
Elispot assay	Identification of CD4+ and CD8+	Not standardized

MHC: Major histocompatibility complex; HLA: Human leukocyte antigen.

Citation: Costa C, Saldan A, Cavallo R. Evaluation of virus-specific cellular immune response in transplant patients. World J Virol 2012; 1(6): 150-153
URL: https://www.wjgnet.com/2220-3249/full/v1/i6/150.htm
DOI: https://dx.doi.org/10.5501/wjv.v1.i6.150