Identification of key genes involved in post-traumatic stress disorder: Evidence from bioinformatics analysis

Table 1 Gene ontology annotations of differentially expressed genes associated with post-traumatic stress disorder (top 5)

Expression	Category	Term	Function	Count	P value
Up-regulated	BP	GO: 0007399	Nervous system development	25	1.21E–08
	BP	GO: 0007409	Axonogenesis	14	1.82E–08
	BP	GO: 0007268	Chemical synaptic transmission	16	1.33E–07
	BP	GO: 0030154	Cell differentiation	32	3.75E–06
	BP	GO: 0010976	Positive regulation of neuron projection development	12	1.51E–05
	CC	GO: 0045202	Synapse	43	1.25E–18
	CC	GO: 0043025	Neuronal cell body	39	1.18E–14
	CC	GO: 0030425	Dendrite	37	2.35E–14
	CC	GO: 0030054	Cell junction	44	7.70E–14
	CC	GO: 0030424	Axon	31	3.10E–13
	MF	GO: 0005515	Protein binding	99	3.15E–05
	MF	GO: 0050839	Cell adhesion molecule binding	8	1.71E–04
	MF	GO: 0019904	Protein domain specific binding	15	3.48E–04
	MF	GO: 0005216	Ion channel activity	11	5.51E–04
	MF	GO: 0008144	Drug binding	8	0.002625
Down-regulated	BP	GO: 0015031	Protein transport	38	7.01E–06
	BP	GO: 0007049	Cell cycle	38	1.59E–05
	BP	GO: 0016192	Vesicle-mediated transport	17	4.14E–04
	BP	GO: 0007040	Lysosome organization	7	7.61E–04
	BP	GO: 0006281	DNA repair	21	8.39E–04
	CC	GO: 0005737	Cytoplasm	243	1.19E–08
	CC	GO: 0005634	Nucleus	219	2.55E–07
	CC	GO: 0005829	Cytosol	77	5.42E–05
	CC	GO: 0005768	Endosome	30	4.60E–04
	CC	GO: 0005794	Golgi apparatus	53	4.91E–04
	MF	GO: 0046872	Metal ion binding	135	4.24E–06
	MF	GO: 0005515	Protein binding	156	1.17E–05
	MF	GO: 0003676	Nucleic acid binding	61	1.93E–05
	MF	GO: 0005487	Nucleocytoplasmic transporter activity	5	0.001069
	MF	GO: 0016874	Ligase activity	22	0.001335

BP: Biological processes; CC: Cellular component; MF: Molecular function; GO: Gene ontology.

Table 2 Kyoto Encyclopedia of Genes and Genomes analysis of differentially expressed genes associated with post-traumatic stress disorder (top 10)

Expression	Term	Definition	Count	P value
Upregulated	mmu04080	Neuroactive ligand-receptor interaction	16	2.02E–05
	mmu05014	ALS	6	1.08E–04
	mmu04530	Tight junction	8	1.23E–03
	mmu00430	Taurine and hypotaurine metabolism	2	1.30E–03
	mmu04020	Calcium signaling pathway	9	2.08E–03
	mmu00650	Butanoate metabolism	3	3.77E–03
	mmu04512	ECM-receptor interaction	5	6.91E–03
	mmu05322	Systemic lupus erythematosus	7	8.98E–03
	mmu04720	Long-term potentiation	4	1.25E–02
	mmu04010	MAPK signaling pathway	10	1.31E–02
Downregulated	mmu05223	Non-small cell lung cancer	9	1.48E–05
	mmu05212	Pancreatic cancer	10	2.35E–05
	mmu05160	Hepatitis C	14	4.31E–05
	mmu05211	Renal cell carcinoma	8	6.64E–04
	mmu05221	Acute myeloid leukemia	7	6.55E–04
	mmu04012	ErbB signaling pathway	9	6.19E–04
	mmu04620	Toll-like receptor signaling pathway	10	5.05E–04
	mmu03013	RNA transport	14	3.89E–04
	mmu05214	Glioma	8	3.55E–04
	mmu05215	Prostate cancer	10	1.91E–04

ALS: Amyotrophic lateral sclerosis; ECM: Extracellular matrix.

Table 3 The most significantly enriched pathways of differentially expressed genes associated with post-traumatic stress disorder

Term	Pathway	P value	Genes
			Upregulated	Downregulated
mmu04010	MAPK signaling pathway	1.51E–08	Crkl, Fgf12, Mras, Mapt, Ntrk2, Dusp8, Ptpn5, Map3k4, Mapk8ip2, Dusp7	Chuk, Egf, Egfr, Ikbkg, Kras, Nfkb1, Prkca, Pla2g4a, Sos1, Map3k8, Stk3, Tab2, Dusp16, Raf1, Taok1
mmu04014	Ras signaling pathway	1.84E–07	Fgf12, Grin2b, Mras, Pgf, Ptpn11, Gng13	Chuk, Egf, Egfr, Gab1, Ikbkg, Insr, Kras, Nfkb1, Pik3ca, Prkca, Pla2g4a, Sos1, Rassf5, Tbk1, Raf1, Rab5a
mmu04012	ErbB signaling pathway	3.85E–06	Camk2b, Cdkn1b, Crkl	Egf, Egfr, Gab1, Kras, Pik3ca, Prkca, Sos1, Tgfa, Raf1