Preterminal protein, the achilles heel of adenoviridae: Implications for adenoviral infections

Figure 1 Adenovirus 2 replication machinery. Adenoviral polymerase, preterminal protein and DBP (+ adenosine triphosphate) are essential for the replication process. Ad: Adenovirus; AdPol: Adenoviral polymerase; ATP: Adenosine triphosphate; PTP: Preterminal protein; NF: Host nuclear factor; Mu: Viral core protein; TP: Terminal protein.

Figure 2 Proposed mechanism (abridged) of adenoviral replication. //: Some steps omitted for clarity.

Figure 3 MALDI-TOFF MS of unbound preterminal protein. Inset (A) shows a 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis chromatogram of pooled unbound and bound preterminal protein (1:1). Preterminal protein (30 µg) appears at 76.283 kDa.

Figure 4 Selectivity of the recombinant endo-O-deglycosidase (spec. activity: ≥ 12 U/mg) for unsubstituted Gal β(1-3)GalNAc-α1 disaccharides O-linked to Ser/Thr residues of glycopeptides (Cleavage site: |).

Figure 5 Elution profiles of recombinant preterminal protein with respect to the ssDNA column. Experimental values are means of triplicate experiments.

Figure 6 Tobacco etch virus protease 7 amino acid recognition site. Cleavage site: -x-Gln⤓ Ser-R₁.

Figure 7 Potential sites a, b, c of direct inhibition, as a result of glycosylation, during initiation of adenoviral replication. Arrows 1 and 2 signify the jumping back step of the CAT trinucleotide at the 5’end of the nascent strand (refer to mechanism in text).