Oxidative stress and labile plasmatic iron in anemic patients following blood therapy

Table 1 Subject characteristics

Characteristics	Controls(n = 15)	Transfusions
		< 5(n = 14)	5-10(n = 11)	> 10(n = 14)
Age (yr)	40.1 (20–50)	62.8 (24–92)	64.8 (49–84)	57.5 (24–74)
Number of transfusions	0 (0)	3.20 (2–4)	7.17 (5–9)	18.78 (14–26)
Hemoglobin (g/dL)	13.8 ± 0.5	7.5 ± 2.1	6.75 ± 0.5	4.9 ± 0.9
Labile iron content (μg/dL)	108.9 ± 13.8	149.2 ± 45.1	216.2 ± 68.3a	366.9 ± 68.5a
Ferritin (μg/L)	219.6 ± 18.2	190.3 ± 11.7	221.2 ± 16.1	277.5 ± 27.5a

Values are presented as median (range), or mean ± standard deviation;

^aP < 0.05 vs control.

Table 2 Spearman’s correlations between biochemical and oxidative markers in polytransfused subjects

	LPI	Ferritin	GPx	SOD	CAT	TBARS	DCHF-DA oxidation	Carbonyl	Total thiol
Transfusion number	0.8569b	0.7991b	-0.8796b	-0.7103b	-0.8143b	0.5114b	0.0111	0.5793b	-0.5555b
Total thiol	-0.4151b	-0.3354b	0.4830b	0.4849b	0.5401b	-0.2790b	0.0106	-0.1164	-
Carbonyl	0.5583b	0.5122b	-0.5613b	-0.3713b	-0.4862b	0.5208b	-0.0627	-	-
DCHF-DA oxidation	-0.1291	-0.0049	0.0370	-0.0446	-0.0401	-0.2293a	-	-	-
TBARS	0.4984b	0.4144b	-0.4638b	-0.3113b	-0.3457b	-	-	-	-
CAT	-0.7266b	-0.5944b	0.8945b	0.7251b	-	-	-	-	-
SOD	-0.6085b	-0.5744b	0.7443b	-	-	-	-	-	-
GPx	-0.7973b	-0.7144b	-	-	-	-	-	-	-
Ferritin	0.9112b	-	-	-	-	-	-	-	-

^aP < 0.05,

^bP < 0.001 vs DCHF-DA oxidation. CAT: Catalase; DCHF-DA: 2’,7’-dichlorodihydrofluorescein diacetate; GPx: Glutatione peroxidase; LPI: Labile plasmatic iron; SOD: Superoxide dismutase; TBARS: Thiobarbituric acid-reactive substances.